PCR Project
This is my lab report on this project
Purpose:
The purpose of this lab was to familiarize ourselves with the PCR process and how it works.
Hypothesis:
I think that if we do it right then the results will be legible, accurate, and there will be a result for every person.
Procedure:
Note PCR Lab Procedure
Data:
The results that we got were very minimal. Only some of the people's DNA with the dye glowed when we put it under
Observations:
These are some of the observation that noticed in this lab…
Analysis:
The fact that not everyone got results in the PCR lab means that we did something wrong. There are many things that we could have done wrong because there was a lot of steps to this lab. One of the things that we could have done wrong was at the end when we were pipetting the dye mixed with our DNA and a lot of people didn't pipet it perfectly into the slots so that might have led to the results not coming in correctly.
The observations that I noted are important because the specific measurements are a very important part of this lab. If you don't measure things correctly then you risk the lab not working or even breaking a centrifuge if your sample is too heavy or too light. The saline solution is also important because if it was just water then not enough skin particles would be picked up and be able to be processed.
Ways to make this lab better would be to explain how these steps are relevant and how they relate to the final product. For example with the measurements explain what the thing you're putting in is, why it is important, and why do you have to put it in.
The reason we did this lab was to familiarise us with PCR and how to see how you test DNA and and also how to do it properly so that if we do it again we will do it right. Our evidence was the picture that we had of our results and the observation noted above. This data shows that some people didn’t do the lab correctly because there are not lines on all the segments where we put the DNA and dye mix.
Purpose:
The purpose of this lab was to familiarize ourselves with the PCR process and how it works.
Hypothesis:
I think that if we do it right then the results will be legible, accurate, and there will be a result for every person.
Procedure:
Note PCR Lab Procedure
Data:
The results that we got were very minimal. Only some of the people's DNA with the dye glowed when we put it under
Observations:
These are some of the observation that noticed in this lab…
- The dye that we used was a very dark blue
- The dye only glowed in the UV light
- The saline solution tasted super bad
- The measurements were very small. All under 50 micro liters
Analysis:
The fact that not everyone got results in the PCR lab means that we did something wrong. There are many things that we could have done wrong because there was a lot of steps to this lab. One of the things that we could have done wrong was at the end when we were pipetting the dye mixed with our DNA and a lot of people didn't pipet it perfectly into the slots so that might have led to the results not coming in correctly.
The observations that I noted are important because the specific measurements are a very important part of this lab. If you don't measure things correctly then you risk the lab not working or even breaking a centrifuge if your sample is too heavy or too light. The saline solution is also important because if it was just water then not enough skin particles would be picked up and be able to be processed.
Ways to make this lab better would be to explain how these steps are relevant and how they relate to the final product. For example with the measurements explain what the thing you're putting in is, why it is important, and why do you have to put it in.
The reason we did this lab was to familiarise us with PCR and how to see how you test DNA and and also how to do it properly so that if we do it again we will do it right. Our evidence was the picture that we had of our results and the observation noted above. This data shows that some people didn’t do the lab correctly because there are not lines on all the segments where we put the DNA and dye mix.